学　术

　　为搭建我校博士后之间的学术交流平台，促进学术水平提升，学校博士后管理办公室组织开展博士后学术沙龙活动。本次沙龙由我校博士后马燕勤分享其研究成果，诚挚邀请感兴趣的师生参加。

　　一、时　间：2021年12月27日（周一）13：00

　　二、地　点：沙河校区主楼西304会议室

　　三、主办单位：电子科技大学博士后管理办公室

　　四、承办单位：生命科学与技术学院　电子科技大学博士后联谊会

　　五、报告简介

　　（1）主　题：Editing activity detection of different tRNA in CRISPR/Cas9 system

　　（2）主讲人：马燕勤  生命科学与技术学院博士后

　　（3）交流内容：

　The bacterial type II clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR associated (Cas) protein system have found widespread utility as tools for site specific DNA and RNA recognition. The most popular Streptococcus pyogenes Cas9 (SpCas9) recognizes a target site containing an NGG protospacer adjacent motif (PAM). Cas9-based tools have been rapidly developed for genome/epigenome editing, transcriptional regulation, and other applications in genetic engineering. Transfer RNAs (tRNAs) are ribonucleic acids that recognize the genetic code of messenger RNAs (mRNAs) and bring amino acids to ribosomes during the process of translation. tRNAs are indispensable molecules in protein synthesis. The secondary structure of a typical tRNA consists of four stems and four loops including D-loop, anticodon loop, variable loop and TΨC loop. tRNA sequencings in various plants reveal that tRNA expression profiles exhibit a cross-species conserved pattern. tRNA was found in the polycistronic transcription unit in bacteria and occasionally in eukaryotes, suggesting that the tRNA-processing system is likely used as an intrinsic mechanism to produce different small RNAs [for example, small nucleolar RNA (snoRNA)] with tRNA from a single polycistronic gene. tRNA is a fundamental cellular component, and its production is guaranteed by the conserved and precise tRNA-processing systems in different organisms. Arrayed tRNA–gRNA architecture was efficiently and precisely processed into gRNAs with desired 5′ targeting sequences in vivo, which directed Cas9 to edit multiple chromosomal targets. The purpose of this study was to examine the editing activity of different tRNA in CRISPR/Cas9 system. The main contents include:1) Effects of pre-tRNA on editing efficiency of CRISPR/Cas9 system; 2) Effects of different expression abundance of tRNA that transporting the same amino acid on CRISPR/Cas9 system editing efficiency; 3)Effects of high expression of tRNA derived from Arabidopsis thaliana and rice on editing efficiency of CRISPR/Cas9 system; 4) Effects of tRNA come from different plants on editing efficiency of CRISPR/Cas9 system; 5) Effects of different tRNA structures on editing efficiency of CRISPR/Cas9 system; 6) Application of different tRNA-CRISPR/Cas9 systems in plants.

　　（4）主讲人简介：

　　Yanqin Ma received the Master degree and Ph.D. degree from the Northwest University in 2016 and 2019, respectively. She currently works as a Postdoctoral Fellow in the school of life science and technology of UESTC. Her research interests include effects of tRNA on CRISPR/Cas9 system editing efficiency; application of different tRNA-CRISPR/Cas9 systems in plants.

　　　　　　　　　　　　　　　　　　　　　　　　　电子科技大学博士后管理办公室

　　　　　　　　　　　　　　　　　　　　　　　　　　　2021年12月24日